The relative abundances of mRNAs of interest were quantified by real-time reverse transcription polymerase chain reaction (PCR) (qPCR). The mRNA was extracted with Qiagen spin columns (Qiagen), and cDNA was transcribed with the GoScript Reverse Transcription System (Promega) according to the manufacturer’s instructions. SYBR Green or TaqMan primers (Applied Biosystems) were used for qPCR analysis (see table S1 for further details).

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