Tumor cells were seeded in multiple 96-well plates at an initial density of 1.5 × 103 to 3 × 103 cells per well (depending on the cell line) and subsequently allowed to grow in complete culture medium with 10% FBS. Every 24 hours, one multiwell dish was fixed with 11% glutaraldehyde and stained with crystal violet, and the absorbance was read at 595 nm.

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