The GSEA software was downloaded from The input data for the GSEA were the following: (i) a complete table of gene expression in get file format, (ii) cld files indicating sample grouping, and (iii) a catalog of functional gene sets from Molecular Signature Database corresponding to hallmarks of cancer (MSigDB version 3.0; 30 September 2010 release;; a total of 50 curated gene sets were included in the analysis. Default parameters were used to compare Sema4C versus mock cells lines, in reference to the hallmark signature database. Inclusion gene set size was set between 15 and 500, and the genes were permutated 1000 times ( (57, 58).

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