VSMCs and skin fibroblasts were cultured in 12-well plates at 5000 cells per well in 2 ml DMEM containing 10% FBS. Twenty-four hours after plating, cells were treated with PDGF (20 ng/ml). RU360 (100 nM) was added with PDGF to some wells. After 24 to 72 hours, cells were trypsinized and counted in triplicate using a Beckman Coulter Z1 cell counter.

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