Antibodies directed against HA (3724), ATP1A1 phospho-Ser16 (4020S), β-actin (4970S), polyclonal normal IgG (2729S), AMPK (2532), AMPK phospho-Thr172 (2535), pan-Akt (4685S), Akt phospho-Thr308 (5056S), Akt phospho-Ser473 (4058), Src (2109S), Src phospho-Tyr416 (2101), EGFR (4267), EGFR phospho-Tyr1068 (8543S), p44/42 MAPK (Erk1/2) (4695), p44/42 MAPK (Erk1/2) phospho-Thr202/Tyr204 (4094S), and anti-Ki-67 (11882) were purchased from Cell Signaling Technology. Antibodies directed against c-myc-tag (MA1980) and ATP1A1 (MA3-928) were purchased from Thermo Fisher Scientific. The antibody directed against mouse normal IgG (sc-2025) was purchased from Santa Cruz Biotechnology. Pepducins and pNaKtide were custom-synthesized by Peptide 2.0 Inc.

The pEXPR-IBA 103 Strep-tag plasmid (2-3503-000) was purchased from IBA Lifesciences. HA-tagged plasmids (GPR035TN00, CXCR20TN00, and CCR050TN00) were purchased from the cDNA Resource Center. Plasmids for ATP1A1 (RC201009), ATP1A2 (SC119715), and ATP1A3 (RC203198) were purchased from OriGene. mVenusN1 (27793) and mCeruleanN1 (27795) were purchased from Addgene. The HPSI0114i-kolf_2 (KOLF2) (ECACC 77650100) iPSC cell line has been previously established by the Wellcome Trust Sanger Institute. Other cell lines were purchased from the American Type Culture Collection.

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