Cell membrane potential was measured by incubating cells with 1.9 μM DiBAC4(3) (Molecular Probes, B438) for 20 min. A fluorescent plate reader was then used to excite the dye at 493 nm and measure emission at 516 nm. ΔΨm was measured by incubating cells with JC-1 (10 μg/ml) (Molecular Probes, T3168) for 10 min at 37°C before measuring emission at 530 nm and 535 nm upon excitation at 485 and 535 nm, respectively.

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