Murine BMDMs and iPSC-derived macrophages were plated in 96-well plates at a density of 50,000 cells per well. After 24 hours, medium was removed and replaced with 100 μl per well of RPMI 1640, 1% l-glutamine, 1% penicillin/streptomycin, and 10% dialyzed FBS (Gibco, 26400044). At set time points, 5 μl of media was then sampled and diluted in 95-μl PBS. Lactate concentration was measured using the Lactate-Glo Assay (Promega, J5021) according to the manufacturer’s instructions.

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