Overnight serum-starved BMDMs were exposed to test compounds for 5 s, 30 s, 5 min, 30 min, and 60 min. Thereafter, cells were immediately lysed in ice-cold lysis buffer containing protease inhibitors, sodium fluoride, sodium pyrophosphate, p-nitrophenyl phosphate, and microcystin LR, and G-LISA assays were performed according to the manufacturer’s instructions. Plates were read at 490 nm, and values are given as extinction coefficient.

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