Mouse femurs and tibias were flushed with macrophage culture medium [RPMI 1640 containing 100 U/ml of penicillin/streptomycin, 1 mM Hepes (pH 7.4), and 10% fetal bovine serum (FBS)], and the bone marrow was filtered through a 70-μm cell strainer. Cells were thereafter incubated in macrophage culture medium supplemented with macrophage colony-stimulating factor (M-CSF) (100 ng/ml) for 6 days. BMDMs were then reseeded and polarized overnight toward M1 or M2 with IFN-γ (50 ng/ml) plus LPS (20 ng/ml) or with IL-4 (20 ng/ml), respectively.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.