BrdU (2.5 mg) was injected intraperitoneally 24 hours before euthanizing mice. Paraffin-embedded samples were then deparaffinized in xylene and dehydrated with ethanol. Cells positive for BrdU were detected using a BrdU In-Situ Detection Kit (BD Pharmingen, 550803). Proliferation was revealed as BrdU+ cells along the crypt-villus axis.

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