MGE or cortex explants were prepared from E14 CD-1 mice without any treatment or E18 CD-1 mouse embryos after intraperitoneal injection with TAT or TAT-D1Rpep into the pregnant mice from E12 to E17. Small tissue fragments corresponding to the subventricular zone of the MGE or cortex were dissected out, placed in a three-dimensional Matrigel gel matrix (catalog number: 356237, BD Biosciences, San Jose, CA), and cultured for 24 hours in Neurobasal medium supplemented with B27 and GlutaMAX in six-well plates (Falcon) at 37°C in a 5% CO2 incubator, as previously described (66) . For explants from E14 CD-1 mice, saline, TAT or TAT-D1Rpep (10 μM) was added into the culture medium right after the explants were placed in to the plates. After 24 hours, MGE explants were imaged at ×10 magnification on an Olympus FV10i confocal microscope.

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