MGE or cortex explants were prepared from E14 CD-1 mice without any treatment or E18 CD-1 mouse embryos after intraperitoneal injection with TAT or TAT-D1Rpep into the pregnant mice from E12 to E17. Small tissue fragments corresponding to the subventricular zone of the MGE or cortex were dissected out, placed in a three-dimensional Matrigel gel matrix (catalog number: 356237, BD Biosciences, San Jose, CA), and cultured for 24 hours in Neurobasal medium supplemented with B27 and GlutaMAX in six-well plates (Falcon) at 37°C in a 5% CO2 incubator, as previously described (66) . For explants from E14 CD-1 mice, saline, TAT or TAT-D1Rpep (10 μM) was added into the culture medium right after the explants were placed in to the plates. After 24 hours, MGE explants were imaged at ×10 magnification on an Olympus FV10i confocal microscope.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.