Six- to 8-week-old mice were injected intraperitoneally with AOM (12.5 mg/kg) (Sigma-Aldrich). Colitis was induced by two cycles of 2.5% DSS (MP Biomedicals) in drinking water for 5 days, followed by a 16-day tap water period (75). The final DSS cycle (2%) was administered for 4 days, followed by a 10-day tap water period. Tumor count and tumor area were determined at day 61 microscopically in longitudinally cut and formalin-fixed specimen.

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