Apcmin/+ mice were obtained from the Jackson Laboratory and intercrossed with mice lacking Gpr35 globally or conditionally in their IECs. At the age of 15 weeks, mice were euthanized, and after flushing with phosphate-buffered saline (PBS), the intestine was longitudinally cut and formalin-fixed, and tumor count was assessed microscopically. Samples were embedded as Swiss rolls in either paraffin or optimal cutting temperature compound for further analysis.

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