For silencing of class I HDACs (13), Rh30 cells were transfected with 100 nM HDAC1 (L-003493-00-0005, 5 nmol), HDAC2 (L-003495-02-0005, 5 nmol), HDAC3 (L-003496-00-0005, 5 nmol), or HDAC10 (L-004072-00-0005, 5 nmol) SMARTpool siRNA reagent (a pool of four siRNA duplexes all designed to target distinct sites within the specific gene of interest) (Dharmacon) versus scrambled siRNA (Dharmacon) using Lipofectamine RNAimax (Invitrogen, catalog no. 13778030). siRNA cell lysates were subjected to Western blotting using anti-HDAC1 (H-51, 1:400; Santa Cruz Biotechnology), anti-HDAC2 (C-8, 1:400; Santa Cruz Biotechnology), anti-HDAC3 (B-12, 1:400; Santa Cruz Biotechnology), and anti-HDAC10 (E-2, 1:400; Santa Cruz Biotechnology) antibodies and PAX3:FOXO1 expression using anti-PAX3 antibody (R&D Systems).

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