For silencing of class I HDACs (13), Rh30 cells were transfected with 100 nM HDAC1 (L-003493-00-0005, 5 nmol), HDAC2 (L-003495-02-0005, 5 nmol), HDAC3 (L-003496-00-0005, 5 nmol), or HDAC10 (L-004072-00-0005, 5 nmol) SMARTpool siRNA reagent (a pool of four siRNA duplexes all designed to target distinct sites within the specific gene of interest) (Dharmacon) versus scrambled siRNA (Dharmacon) using Lipofectamine RNAimax (Invitrogen, catalog no. 13778030). siRNA cell lysates were subjected to Western blotting using anti-HDAC1 (H-51, 1:400; Santa Cruz Biotechnology), anti-HDAC2 (C-8, 1:400; Santa Cruz Biotechnology), anti-HDAC3 (B-12, 1:400; Santa Cruz Biotechnology), and anti-HDAC10 (E-2, 1:400; Santa Cruz Biotechnology) antibodies and PAX3:FOXO1 expression using anti-PAX3 antibody (R&D Systems).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.