Chemically synthesized double-stranded siRNA duplexes (with 3′-dTdT overhangs) were obtained from Qiagen for the following targets: GRK2 (5′-AAGAAAUUCAUUGAGAGCGAU-3′) and GRK3 (5′-AAGCAAGCUGUAGAACACGUA-3′), and a non-silencing RNA duplex (5′-GCUUAGGAGCAUUAGUAAA-3′ and 3′-UUUACUAAUGCUCCUAAGC-5′) was obtained from GE Dharmacon. Stably HA-hNOP receptor–expressing HEK293 cells were transfected with 150 nM siRNA for single transfection or with 100 nM of each siRNA for double transfection for 3 days using HiPerFect. All experiments showed target protein abundance reduced by ≥80%.

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