Animal experiments were performed in accordance with the approved Institutional Animal Care and Use Committee protocol at Weill Cornell Medicine. Age- and gender-matched C3H/HeJ mice (female, 7 weeks old) were purchased from the Jackson laboratory. For the analysis of epidermal pigmentation, C3H/HeJ mouse ears were topically treated with 20 μl of KH7 (42 mg/ml) or LRE1 (28 mg/ml) on the right ear, and DMSO (vehicle control) was topically applied on the left ear twice a day for 2 weeks. In parallel, a different group of mice was treated with DMSO on both ears. Ear skin was monitored daily for irritation and changes in pigmentation. After the final treatment, mice were euthanized, and the treated skin was submitted to an animal pathologist for blinded histological evaluation of the epidermis and specific staining (Fontana-Masson). This experiment was performed twice with three mice per cohort (for a total of six mice).

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