The NOP receptor agonist AT-202 was synthesized and provided by Astraea Therapeutics. Ro64-6198 was provided by Roche. Nociceptin (ab120070) and dynorphin A (ab120412) were obtained from Abcam. MCOPPB (PZ0159), ß-endorphin (E6261), DADLE (E7131), DAMGO (E7384), etonitazene (E5007), etorphine, fentanyl (F3886), hydromorphone (H5136), [Met]-enkephalin (M6638), morphine-6-glucuronide (M3528), oxycodone (O1378), pentazocine (P134), tapentadol (T058), naloxone (N7758), and PMA (P8139) were purchased from Sigma-Aldrich. SCH221510 (3240), NNC 63-0532 (1780), J-113397 (2598), SB 612111 (3573), DPDPE (1431), endomorphin-1 (1055), endomorphin-2 (1056), U50488 (0495), PTX (3097), SCH23390 (0925), and forskolin (1099) were obtained from Tocris. Norbuprenorphine (BUP-982-FB) and buprenorphine (BUP-399-HC) were purchased from Lipomed. γ-Endorphin (60893-02-9) was obtained from Bachem, and tramadol was obtained from Grunenthal. Levomethadone (00424906) and pethidine (03012446) were purchased from Sanofi-Aventis. Morphine (26-6) was obtained from Merck Pharma. Nalfurafine (A12579) was purchased from AdooQ Bioscience LLC. Piritramid and sufentanil were obtained from Hameln Pharma Plus. Remifentanil was purchased from GlaxoSmithKline. Nortilidine was obtained from Pfizer AG. Cebranopadol (HY-15536) was purchased from MedChem Express. λ-Phosphatase (P0753S) was obtained from Santa Cruz Biotechnology. Compound 101 (HB2840) was obtained from Hello Bio. Tertiapin-Q was purchased from Alomone Labs. AT-202, Ro64-6198, PMA, SCH221510, NNC 63-0532, J-113397, SB 612111, DPDPE, U50488, forskolin, nalfurafine, and cebranopadol are DMSO soluble, and all the other mentioned compounds are water soluble.

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