Tyrosinase antibody (used at 1:1000) was a gift from R. Halaban, Yale University School of Medicine (85). Tyrosinase antibody (T311) was purchased from Santa Cruz Biotechnology (1:200; catalog no. sc-20035, lot no. J0616). MITF antibody was purchased from Abcam (1:500; catalog no. ab13703, lot no. GR27425-10). MITF (D5G7V; 1:1000; catalog no. 12590S, lot no. 1), phospho-(Ser/Thr) PKA substrate (1:1000; catalog no. 9621, lot no. 14), and GAPDH (14C10; 1:1000; catalog no. 2118S, lot no. 8) antibodies were purchased from Cell Signaling Technology. Antibodies directed against (R21) (3.2 mg/ml, 1:2000) were used as previously described (28).

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