All procedures were essentially performed as previously described (26, 30, 52). Briefly, tumor tissues were fixed in Z-fix (zinc-buffered formaldehyde, Anatech Ltd.) overnight and then transferred to 70% ethanol. Fixed tissues were embedded in paraffin, sectioned to a thickness of 4 μm, and stained with hematoxylin and eosin. Histology slides were scanned with a Scanscope Digital microscope (Aperio).

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