Cells were cultured on poly-l-lysine–coated coverslips and fixed with 4% PFA in PBS. Cells were permeabilized with 0.1% Triton X-100 for 5 min at 4°C, which was followed by blocking in PBS, 0.5% bovine serum albumin (BSA) for 30 min at room temperature. After blocking, cells were incubated in blocking solution (PBS, 0.5% BSA) containing an anti-YAP primary antibody (Santa Cruz Biotechnology) overnight at 4°C. The next day, cells on the coverslips were visualized with Alexa Fluor–labeled secondary antibodies (Thermo Fisher Scientific) and 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) for nuclear staining. Cells were mounted on glass slides with Shandon Immu-Mount (Thermo Fisher Scientific).

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