Cells were cultured on poly-l-lysine–coated coverslips and fixed with 4% PFA in PBS. Cells were permeabilized with 0.1% Triton X-100 for 5 min at 4°C, which was followed by blocking in PBS, 0.5% bovine serum albumin (BSA) for 30 min at room temperature. After blocking, cells were incubated in blocking solution (PBS, 0.5% BSA) containing an anti-YAP primary antibody (Santa Cruz Biotechnology) overnight at 4°C. The next day, cells on the coverslips were visualized with Alexa Fluor–labeled secondary antibodies (Thermo Fisher Scientific) and 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) for nuclear staining. Cells were mounted on glass slides with Shandon Immu-Mount (Thermo Fisher Scientific).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.