FR was isolated and purified from the dried leaves of Ardisia crenata, as previously described (14). Reversed-phase high-performance liquid chromatography separation of the FR-containing fraction [column: YMC C18 Hydrosphere, 250 × 4.6 mm, 3 μm; MeOH:H2O (8:2), 0.7 ml min−1] yielded FR of 95% purity.

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