Gli2ΔN cells were lysed with radioimmunoprecipitation assay buffer (sc-24948, Santa Cruz Biotechnology) supplemented with Halt Protease and Phosphatase Inhibitor (no. 1861218, Thermo Fisher Scientific) for 30 min on ice, and lysates were cleared by centrifugation at 13,000 rpm for 15 min at 4°C. Supernatants were incubated with 6× Laemmli sample buffer (10570021-1, BioWorld) at 95°C for 5 min. The samples were then separated with a 7% SDS-PAGE gel and immunoblotted with anti-Gli2 (AF3635-SP, R&D Systems) and anti–β-tubulin (ab6046, Abcam).

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