Male αCaMKII-Cre or vGAT-IRES-Cre mice (7 to 8 weeks) were anesthetized with ketamine/xylazine solution (130 and 10 mg/kg) and mounted on a stereotaxic frame. The hippocampal CA1 region was targeted using the following coordinates: anterior-posterior (AP): −1.8 mm, medial-lateral (ML): ±1.0 mm, dorsal-ventral (DV): −1.7 mm/AP: −2.5 mm, ML: ±2 mm, DV: −1.8 mm. AAV (0.5 μl of 4 × 1012 vg/ml) was injected into each point. All mice were allowed to recover for a minimum of 3 weeks before further use in experiments. Experimenters were blinded to the type of viral vector injected.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.