Cells were infected with VSV for 6 hours, and total RNA was purified using the RNeasy Mini Kit (Qiagen no. 74104). The transcriptome library for sequencing was generated using the VAHTSTM mRNA-seq v2 Library Prep Kit for Illumina (Vazyme Biotech Co. Ltd., Nanjing, China) following the manufacturer’s recommendations. After clustering, the libraries were sequenced on Illumina HiSeq X Ten platform using (2 × 150 base pairs) paired-end module. The raw images were transformed into raw reads by base calling using CASAVA (http://support.illumina.com.cn/sequencing/sequencing_software/casava.html). Then, raw reads in a fastq format were first processed using in-house perl scripts. Clean reads were obtained by removing reads with adapters, reads in which unknown bases were more than 5% and low-quality reads (the percentage of low-quality bases was more than 50% in a read; we defined the low-quality base to be the base whose sequencing quality was no more than 10). At the same time, Q20, Q30, and GC content of the clean data were calculated (Vazyme Biotech Co. Ltd., Nanjing, China). The original data of the RNA-seq were uploaded to the Gene Expression Omnibus (GEO) DataSets (GEO accession nos. GSE107174 and GSE120820).

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