Total RNA was isolated from cells using the RNeasy RNA Extraction Kit (Qiagen), and cDNA synthesis was performed using 1 ug of total RNA (iScript cDNA Synthesis kit). Expression of the indicated genes was analyzed by qRT-PCR amplified using SYBR Green (Transgene). Data shown are the relative abundance of the indicated mRNA normalized to Gapdh. The specific primers are listed in table S2.

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