Resolving gels were blotted to an Immobilon-FL polyvinylidene difluoride (PVDF) membrane (Millipore). Membranes were blocked with the Odyssey Blocking Buffer (LiCor Biosciences) diluted 1:1 with PBS and probed with either a 1:5000 dilution of rabbit 6325 antiserum for GC-A Western blots or rabbit 6327 antiserum for GC-B Western blots followed by 1:15,000 goat anti-rabbit IRDye 680–conjugated secondary antibody (LiCor Biosciences). FLAG Western blots were probed using a 1:5000 dilution of FLAG-M2 monoclonal antibody (Sigma-Aldrich) followed by 1:15,000 goat anti-mouse IRDye 800–conjugated secondary antibody (LiCor Biosciences). Actin Western blots were probed using a 1:2000 dilution of a monoclonal mouse β-actin antibody (Sigma-Aldrich) followed by 1:15,000 goat anti-mouse IRDye 800–conjugated secondary antibody. All Western blots were visualized on a LiCor instrument as described previously (69).

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