Immunoblotting was carried out by standard procedures. Briefly, samples eluted with 4× loading buffer were boiled at 100°C for 5 min and then loaded to gels (SDS-PAGE). After electrophoresis, proteins were transferred to a 0.22-um nitrocellulose membrane (PALL). Membranes were probed with antibodies using standard techniques and detected by enhanced chemiluminescence. The antibodies were diluted 1000 times for immunoblots. Western blotting data were quantified using ImageJ software. Normalized band intensity values were the ratio of bands intensity to corresponding internal reference. For Western blotting–involved cleavage, normalized band intensity values were the ratio of the abundance of the cleaved and uncleaved form to corresponding internal reference.

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