Relative amounts of biologically active IFN in sera were quantified using a 2fGTH-ISRE (human derived) or L929-ISRE (mouse derived) cell line stably expressing an ISRE-luc reporter. Briefly, 200 μl of culture medium was incubated with confluent 2fGTH-ISRE or L929-ISRE cells (24-well plate) for 6 hours. Cells were lysed in passive lysis buffer and subjected to luciferase quantification (Promega). A serial dilution of human IFN-β was included as standards.

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