To induce tumor growth, 6- to 8-week-old female BALB/c mice were injected subcutaneously in the scruff of the neck with 1 × 106 RencaHA cells suspended in PBS. The mice were monitored regularly to assess tumor growth. Tumor size was measured using a caliper and calculated using the following equation: volume (V) = L2 × W/2, where length (L) was the longest measurement and width (W) was measured perpendicularly to length. Mice were culled if tumors exceeded 15 mm in any one direction or any humane end points were reached. GFP-positive clone 4 CTLs were resuspended in PBS at 2 × 107 cells/ml, and 200 μl of cells was injected into the tail vein of a tumor-bearing mouse. Unless otherwise specified, only mice bearing tumors greater than 500 μm3 in volume were used, and tumors were harvested 96 hours after transfer.

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