Stromal vascular preadipocytes from inguinal adipose tissue from ~6-week-old mice were isolated and cultured and differentiated as previously described (32). Where indicated, cells were stimulated with 10 μM forskolin (Sigma-Aldrich) for 4 hours. For autophagic flux assays, cells were starved in serum-free Hank’s balanced salt solution and treated with chloroquine (50 μM) or vehicle for the given times.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.