Stromal vascular preadipocytes from inguinal adipose tissue from ~6-week-old mice were isolated and cultured and differentiated as previously described (32). Where indicated, cells were stimulated with 10 μM forskolin (Sigma-Aldrich) for 4 hours. For autophagic flux assays, cells were starved in serum-free Hank’s balanced salt solution and treated with chloroquine (50 μM) or vehicle for the given times.

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