Cells or tissues were lysed in a standard radioimmunoprecipitation assay lysis buffer. Standard techniques were used for protein quantification, separation, transfer, and blotting. The following primary antibodies were used: Cathepsin D (1:4000; a gift from S. Kornfeld, Washington University), Lamp-1 (1:2000; sc-19992, Santa Cruz Biotechnology), LC3 (1:1000; NB100-2220, Novus Biologicals).

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