GTT and ITT were performed in mice after 2 or 4 months on a WD or at 8 to 9 weeks of age in chow-fed mice. Mice were weighed, transferred to clean cages, and fasted for 5 hours. Mice were injected with 1 g of glucose/g body mass or insulin (0.75 U/kg) for GTT and ITT, respectively. Blood glucose levels were measured immediately before injection and at 30, 60, and 120 min after injection using a glucometer and tail bleed.

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