A minimum of two 20× fields per mouse were imaged in inguinal, gonadal, and brown adipose hematoxylin and eosin (H&E)–stained slides by a blinded observer at identical settings. Images were processed identically to remove background using Adobe Photoshop CS5. Adipocyte sizing analyses were performed using the Adiposoft plugin for ImageJ software. BAT lipid content was assessed by identically thresholding grayscale-converted images to match lipid area and presented as the percentage of total area.

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