Animal protocols were approved by the Washington University Animal Studies Committee. All mice used in this study were on C57BL/6J background (backcrossed at least seven generations). Mice carrying a TFEB transgene allele were as previously described (3, 4, 9). These mice were crossed with Cre-recombinase transgenic mice under the control of the adiponectin promoter to generate Adipo-TFEB mice. For studies examining mice with concurrent adipocyte-specific PGC-1α deficiency, these alleles were crossed into a homozygous PGC-1α flox background (29). TFEB transgene, Cre, and PGC-1α flox allele genotyping was performed using standard polymerase chain reaction (PCR) techniques (table S1). Mice housed in a specific pathogen–free barrier facility were weaned at 3 weeks of age to a standard mouse chow providing 6% calories as fat. All mice were housed at 21°C unless otherwise specified. For diet-induced obesity experiments, mice were fed starting at ~2 months of age with WD: 0.15% cholesterol, 42% calories as fat, and 15% calories as protein (TD 88137, Harlan). In each experiment, only samples from the same sex and similar age were compared.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.