Mouse skin/tumor tissues and human AK/SCC biopsies were fixed with formalin and embedded in paraffin. Tissue sections were analyzed by H&E staining or by immunofluorescence using marker-specific primary antibodies, Alexa Fluor 488/595–conjugated secondary antibodies, and Hoechst 33324. BrdU in tissue sections was analyzed using the BrdU In Situ Detection Kit (BD Biosciences).

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