Keratinocytes from the pinna of 10- to 12-week-old mice were incubated with Hoechst 33324 (5 μg/ml) for 1 hour, washed with ice-cold phosphate-buffered saline, stained with propidium iodide (1 μg/ml), and analyzed using Fortessa X-20 (BD Biosciences) and FlowJo (Tree Star).

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