Y. pseudotuberculosis YPIII yopM- (29) or Y. pseudotuberculosis YPIII cultures were grown overnight in LB and enumerated by Coulter counter (Multisizer 4, Beckman Coulter). Yersinia was diluted in PBS for intraperitoneal delivery of 2000 CFU (Y. pseudotuberculosis YPIII yopM-) or 1000 CFU (Y. pseudotuberculosis YPIII) in 200 μl to 6- to 8-week-old mice. Mice were euthanized on day 4, and spleens were homogenized and plated for CFU.

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