HEK293T cells were cotransfected with packaging plasmid (psPAX2) and envelope plasmid (VSVg) together with a plasmid expressing shRNA specific for mouse Fli1 (TRCN0000324345 for shRNA#1 or TRCN000032413 for shRNA#2, Sigma-Aldrich) or NT control shRNA (Sigma-Aldrich). We transfected HEK293T cells in a 10-cm dish and harvested the virus-containing culture medium 48 hours after changing the transfection medium to DMEM containing 10% FBS. The collected medium was filtered through 0.45-μm filters. The filtered virus were diluted in DMEM containing 10% FBS and used to infect bone marrow cells after 1 day of culture. Twenty-four hours after infection, the medium was replaced with fresh macrophage medium containing puromycin (5 μg/ml). After 72 hours of puromycin selection, the macrophages were replated for further experiments.

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