Primary mouse keratinocytes isolated from the skin of C57BL/6 newborn mice and primary human keratinocytes (a gift from G. P. Dotto) were cultured as described (49, 50). KERT cells (a gift from A. Mandinova; originally from the American Type Culture Collection) and 293T cells were cultured in keratinocyte-SFM and Dulbecco’s modified Eagle’s medium (both from Thermo Fisher Scientific), respectively.

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