Glucose oxidation was measured in cardiac tissue from the AAR as previously published (43). Tissue was incubated at 37°C for 1 hour in modified Krebs-Ringer buffer [115 mM NaCl, 2.6 mM KCl, 1.2 mM KH2PO4, 10 mM NaHCO3, and 10 mM Hepes (pH7.4)] that contained 2% fatty acid–free BSA, 0.6 mM palmitate, 6 mM glucose, and d-[14C(U)]glucose (5 μCi/ml) and was gassed with 95% O2 and 5% CO2. The reaction was terminated, and 14CO2 was released by administration of perchloric acid. Released 14CO2 was trapped in NaOH-soaked Whatman paper that was placed in the central well of the flasks. A PerkinElmer scintillating counter (model Tri-Carb 2810TR) was used for counting.

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