Blood was collected from mice 24 hours post-IR injury. Serum was separated from coagulated blood by centrifugation at 4°C. Serum troponin I levels were measured using the Ultra-Sensitive Mouse Cardiac Troponin-I ELISA (Life Diagnostics, West Chester, PA). The assay was conducted according to the manufacturer’s instructions, and measurements were acquired at 450 nm on a Tecan Infinite M1000Pro plate reader.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.