Blood was collected from mice 24 hours post-IR injury. Serum was separated from coagulated blood by centrifugation at 4°C. Serum troponin I levels were measured using the Ultra-Sensitive Mouse Cardiac Troponin-I ELISA (Life Diagnostics, West Chester, PA). The assay was conducted according to the manufacturer’s instructions, and measurements were acquired at 450 nm on a Tecan Infinite M1000Pro plate reader.

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