HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) penicillin/streptomycin. Cells were transfected with 4 μg of pcDNA3.1 encoding a WT or mutant human KV1.5 (KCNA5) using 10 μl of Lipofectamine 2000 (Thermo Fisher Scientific) and used 24 to 48 hours later.

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