A Seahorse XFe24 analyzer was used to measure mitochondrial OCR and ECAR using the Mito Stress test following a previously published protocol (43). Primary mouse microglia were plated 90,000 cells per well of a 24-well Seahorse plate. All treatments were performed in serum-free medium. For the Mito Stress test, 0.75 μM oligomycin, 1 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone, and 0.5 μM rotenone-antimycin were used. The Seahorse’s Mito Stress and cell phenotype report generators were used for analysis.

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