Cells were plated in 96-well tissue plates. After treatment, 10 μl of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent (Promega) was added and incubated at 37°C for 1.5 hours. After incubation, a plate reader was used to quantify the absorbance at 490 nm.

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