Multiplex cytokine assay was performed according to our previous publication (93). Briefly, 40 μl of treatment medium was incubated overnight with 40 μl of primary antibodies conjugated with magnetic beads. After incubation with primary antibodies, samples were washed, incubated with a biotinylated antibody and biotin-streptavidin, and then read in a Bio-Plex plate reader. Standards were obtained from PeproTech. For welder serum cytokine analysis, the Bio-Plex Pro Human Cytokine 27-plex Assay (M500KCAF0Y, Bio-Rad) was performed according to the manufacturer’s protocol.

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