Eight-week-old male C57BL/6Ncrl mice, obtained from Charles River Laboratories, were housed under standard conditions of constant temperature (22 ± 1°C), humidity (relative, 30%), and a 12-hour light/dark cycle. After acclimating for 3 days, mice were gavaged with Mn (15 mg/kg) for 30 days and then euthanized. This Mn dose regimen was chosen on the basis of previous studies in humans and animals (9496). Use of the animals and protocol procedures were approved by the Institutional Animal Care and Use Committee at Iowa State University (Ames, IA, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.