Age-matched 10- to 12-week-old male WT (n = 15) and homozygote SNAP25Δ3 animals (n = 14) were assessed for their ability to maintain balance upon a rotating cylinder undergoing constant acceleration from 4 to 40 rpm (106). The cylinder was 3 cm in diameter, and each animal was confined to about 6 cm of cylinder length with Plexiglas dividers. Cylinders were suspended 25 cm above a lever that actuates the timer, resulting in a stoppage of the timer to permit tabulation of the latency required for the animal to fall off the cylinder. The maximum length for each trial was 300 s, at which point a maximum latency value of 300 was tabulated. Trials were conducted for three consecutive days. Data for each day were represented as the mean latency to drop from the rotarod (in seconds) ± SE and analyzed using Student’s two-tailed t test for comparisons by day using GraphPad Prism.

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