Open-field activity was tested in age-matched 10- to 12-week-old male WT (n = 15) and homozygote SNAP25Δ3 animals (n = 14) using an open-field system (OFA-510, MedAssociates, St. Albans, VT, USA) with three 16 × 16 arrays of infrared photobeams as previously described (105). Total number of photobeam breaks were collected over 60 min under light or infrared lighting conditions. Data were calculated as either the mean distance traveled (centimeter) ± SE for the number of photobeam breaks/5-min bin/genotype, the total distance traveled (mean horizontal and vertical beam breaks/30 mins), or the total rearing behavior (mean vertical beam breaks/30 mins). Data are presented as means ± SEM and were analyzed by two-way ANOVA (mean distance traveled) and Student’s two-tailed t test (total distance traveled and rearing) using GraphPad Prism.

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