Recombinant proteins were incubated with 17.3 μM S-adenosyl-l-[methyl-14C]methionine (PerkinElmer) and enzyme in methylation buffer [50 mM tris-HCl (pH 8.0), 20 mM KCl, 5 mM MgCl2, 2 mM DTT, 10% glycerol] at 30°C for 2 hours. The samples were run on SDS-PAGE, dried, and exposed to a phosphor screen for 3 days.

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