We used GOrilla to assess whether any GO features are enriched in the GSK591-responding methylated proteins: These were tested against the bulk of the identified methylated proteins for statistically significantly overrepresented GO molecular function, biochemical processes, and cellular component terms (56). The analysis was performed, indicating a P value cutoff of 0.001. We built up the protein network of GSK591-responding methylated proteins using the STRING database (57) and visualized it with Cytoscape (58).

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